BCG and BCGΔBCG1419c protect type 2 diabetic mice against tuberculosis via different participation of T and B lymphocytes dendritic cells and pro-inflammatory cytokines

摘要

T2D mice were fed with a HFD during the whole experiment, and control mice were feed with a balanced diet to control and determine metabolic parameters to confirm T2D. After 2 months of the beginning of HFD, mice progressing to T2D were injected intraperitoneally with 100 mg/kg of STZ to establish the T2D model. Prior to challenge with H37Rv, blood samples were taken for metabolic evaluation. Two and four months after infection, efficacy of protection of both BCG strains was assessed. Mice were weighted weekly to monitor their weight gain before infection, or their weight loss after infection. Data are presented as mean and standard derivation of  = 25 mice per group. Weight gain in mice of the groups prior to infection. Data are presented as median and range of all mice per time. , Weight loss in mice of the groups 2 and 4 months after infection. Glucose concentration of mice was measured at 0, 2, 4, 6, and 8 months to confirm hyperglycemia and the later effect of vaccination and/or infection. Data are presented as median and range of 5 mice per time evaluated. Hemoglobin A1C (A1C) in mice at 2 and 4 months after infection. Data are presented as median and range of 4 mice per time evaluated. As an indicator of insulin resistance, HOMA-IR index was calculated for mice. Median and standard derivation of three calculations of HOMA-IR index is shown. Group comparisons where  p #  t test.