Short-term NAD+ supplementation prevents hearing loss in mouse models of Cockayne syndrome

摘要

Total NAD levels per mg of the cochlea and relative NAD /NADH levels were measured in the cochlea of and WT (6-month-old) mice (  = 3). Two-tailed -tests were used to determine significant difference. Outline for NR treatment and ABR recordings in mice. ABR thresholds for and WT mice following a 10-day treatment with NR (12 mM) delivered via drinking water. A total of 21 mice ( (  = 7); + NR (  = 6); WT (  = 4); WT + NR (  = 4)) were tested by ABR at 5 weeks of age and again following the 10-day treatment (6.5 weeks of age). Multiple graphs are used to visualize error bars on each dataset. Two-way ANOVA with Tukey’s post-hoc test was used to determine significant difference. Treatment with NR (12 mM) prevents the increased threshold shift observed in mice between 5 weeks and 6.5 weeks at 32 kHz. Note: ABR data in at 5 and 6.5 weeks of age were used to calculate the hearing shift. Two-way ANOVA with uncorrected Fisher's LSD post-hoc test was used to determine significant difference.