Key role of MIF-related neuroinflammation in neurodegeneration and cognitive impairment in Alzheimer’s disease

摘要

In-vitro results of STZ stimulation on murine Microglia and Astrocytes. ELISA of MIF secretion in supernatants of astrocytes and microglia after 24 h STZ treatment. Graphs represent the mean of  = 3 biological replicates (two technical replicates for each) with standard errors of the mean (± SEM), (*  b and mRNA expression levels for different cytokine in response to STZ treatment in astrocytes ( ) and microglia ( ) with and without ISO-1 treatment. - . Cytokine ELISA of astrocytes after 24 h STZ treatment. Data are means of  = 3 independent biological replicates, error bars represents ± SEM (*  d), IL-1β ( ) and IL-12p40 ( ) were released in response to STZ treatment in astrocytes using different concentrations of ISO-1 to inhibit MIF resulted in dose dependent decrease in cytokine release in both cell types. Cytokine ELISA in wild type microglia. IL-6 ( ) IL-1β ( ) and IL-10 ( ) were measured after STZ treatment with and without ISO-1. One-way ANOVA with Tukey’s multiple comparison test was performed. (*  P