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Phytocomplex Characterization and Biological Evaluation of Powdered Fruits and Leaves from Elaeagnus angustifolia

机译:桔梗粉状果实和叶片的植物复合物表征及生物学评价

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摘要

Fully ripe fruits and mature leaves of were harvested and analyzed by means of analytical and biological tests to better comprehend the chemical composition and therapeuticutraceutical potential of this plant. Fruits and leaves were dried and the obtained powders were analyzed to study their color character and (via headspace gas chromatography) describe the chemical profile. Subsequently, they were submitted to a chloroform–methanol extraction, to a hydroalcoholic extraction procedure assisted or not by microwaves, and to an extraction with supercritical CO , assisted or not by ethanol as the co-solvent, to detect the polyphenolic and the volatile content. The resulting extracts were evaluated in terms of chlorophyll and carotenoid content, polyphenolic content, volatile fraction, total phenolic content, total flavonoid content, antioxidant activity, radical scavenging activity, and enzymatic inhibition activity. The results confirmed the correlation between the chemical composition and the high antioxidant potential of leaf extracts compared to the fruit extracts in terms of the phenolic and pigment content. A promising effect against tyrosinase emerged for all the extracts, suggesting a therapeuticutraceutical use for this plant. Conversely, the volatile content from both natural matrices was similar.
机译:收获完全成熟的果实和成熟的叶子,并通过分析和生物学测试进行分析,以更好地理解该植物的化学组成和治疗/营养潜力。干燥水果和树叶,分析所得粉末以研究其颜色特征,并(通过顶空气相色谱法)描述其化学特征。随后,他们接受了氯仿-甲醇萃取,采用或不采用微波辅助的水醇萃取程序,以及采用或不采用乙醇作为助溶剂的超临界CO萃取,以检测多酚和挥发性成分。根据叶绿素和类胡萝卜素含量,多酚含量,挥发分,总酚含量,总黄酮含量,抗氧化剂活性,自由基清除活性和酶抑制活性对所得提取物进行评估。结果证实了与水果提取物相比,叶提取物的化学成分与高抗氧化能力之间的相关性在酚和色素含量方面。所有提取物均出现了针对酪氨酸酶的有希望的作用,表明该植物具有治疗/营养用途。相反,两种天然基质的挥发物含量相似。

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