The lre‐miR159a‐LrGAMYB pathway mediates resistance to grey mould infection in Lilium regale

摘要

Grey mould is one of the most determinative factors of lily growth and plays a major role in limiting lily productivity. MicroRNA159 (miR159) is a highly conserved microRNA in plants, and participates in the regulation of plant development and stress responses. Our previous studies revealed that lre‐miR159a participates in the response of to according to deep sequencing analyses; however, the response mechanism remains unknown. Here, lre‐miR159a and its target gene were isolated from . Transgenic overexpressing exhibited larger leaves and smaller necrotic spots on inoculation with than those of wild‐type and overexpressing plants. The overexpression also led to repressed expression of two targets of miR159, and , and enhanced accumulation of hormone‐related genes, including , , , , and for both the jasmonic acid and salicylic acid pathways. Moreover, lower levels of H O and were observed in transgenic , which reduced the damage from reactive oxygen species accumulation. Taken together, these results indicate that lre‐miR159a positively regulates resistance to grey mould by repressing the expression of its target gene and activating a defence response.