Identification of stress resilience module by weighted gene co-expression network analysis in Fkbp5-deficient mice

摘要

Transcriptome analyses of the mPFC in homozygous wild-type (WT) and knock-out (KO) mice following chronic stress. Schematic timeline of the induction of chronic restraint stress. Effect of CRS on sucrose preference. Data were combined with the 1st and 2nd sucrose preference test (SPT) results. Control mice (CT)  = 9; CRS-exposed WT mice (WT_ST)  = 6; CRS-exposed KO mice (KO_ST)  = 6. One-way ANOVA (F[2,39] = 11.67,  = 0.0001); Fisher’s LSD: ***  c Multidimensional scaling (MDS) plot for transcriptomes of individual samples of CT (yellow), WT_ST (blue) or KO_ST (red). The interleaved scatter plots of modules which have a significant negative correlation with stress. Data represent mean ± SEM. One-way ANOVA; Fisher’s LSD: *  ≤ 0.05, **  e A heatmap showing the expression of M55 genes in the mPFC of CT (left), WT_ST (middle) and KO_ST (right). A network plot of M55 genes and their intramodular connections. The ten hub genes (the top ten genes with highest intramodular connectivity; and ) are shown in red. Note their central position in the network, suggesting high intramodular connectivity. Enrichment dot plot for Gene Ontology (GO) analysis of M55 genes. The 13 GO terms with the lowest -value each annotation cluster are plotted in order of gene ratio. The dot size represents the number of genes associated with a specific term. The dot color represents the adjusted p-value