High-yield production of l-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum

摘要

Effect of NCgl2050, NCgl2065, and NCgl0580 deletion and NCgl0580 complement on SSAAI. Cell growth (squares) and -serine titer (circles) of NCgl2050 deletion strain SSAAIΔNCgl2050 (open symbols) and SSAAI (solid symbols). Cell growth (squares) and -serine titer (circles) of NCgl2065 deletion strain SSAAIΔNCgl2065 (open symbols) and SSAAI (solid symbols). Cell growth (squares) and -serine titer (circles) of NCgl0580 deletion strain SSAAIΔNCgl0580 (open symbols) and SSAAI (solid symbols). Cell growth (squares) and -serine titer (circles) of complemented strain SSAAI∆NCgl0580-NCgl0580 (open symbols) and SSAAI (solid symbols). Squares and circles indicate cell growth OD and -serine titer, respectively. The growth rates of the complemented strain SSAAI∆N0580-NCgl0580 (red) and SSAAI (black)