A new transformation method with nanographene oxides of antisense carryingyycG RNA improved antibacterial properties on methicillin-resistantStaphylococcus aureus biofilm

摘要

has the potential to opportunistically causeinfectious diseases. The aim of this study was to determine the antimicrobial effects ofnovel graphene oxide (GO)-polyethylenimine (PEI)-based antisense (AS ) on the inhibition of methicillin-resistant (MRSA) biofilm formation. In current study, a novel GO-PEI-basedrecombinant AS vector transformation strategy was developed toproduce AS . The mechanical features including zeta-potential andparticle size distributions were evaluated by: GO; GO-PEI andGO-PEI-AS . The recombinant AS vector wastransformed into MRSA cells, and the expression levels of the and genes were determined and compared by quantitative real-time PCR(qPCR) assays. The recombinant AS plasmids were subsequently modifiedwith a gene encoding enhanced green fluorescent protein (AS -eGFP) asa reporter gene, and the transformation efficiency was assessed by the fluorescenceintensity. The biofilm biomass and bacterial viability of the MRSA strains were evaluatedby crystal violet assay, colony-forming unit assays and confocal laser scanningmicroscopy. The results showed that the Z-average sizes of GO-PEI-AS were much larger than those of GO or GO-PEI. The GO-PEI-based strategy significantlyincreased the efficiency of AS transformation. TheGO-PEI-AS -transformed MRSA strain had the lowest expression levelsof the biofilm formation-associated genes. Furthermore, GO-PEI-AS suppressed biofilm aggregation and improved bactericidal effects on the MRSA after 24 hrof biofilm establishment. Our findings demonstrated that GO-PEI based antisense RNA will be an effective method for management of MRSAinfections.