Conventional tube culture systems have long been the mainstay in clinical virology for the growth and identification of viruses from clinical specimens. Innovations such as centrifugation-enhanced shell vial and multiwell plate cultures and the use of genetically engineered and mixed cell lines, coupled with faster detection of viral replication, have allowed for reasonable turnaround times for even some of the most slowly growing clinically important human viruses. However, molecular methods, in particular, the PCR, have usurped the role of viral culture in many laboratories, limiting the use of this traditional method of virus detection or replacing it altogether. Advances and improvements in molecular technology over time have also resulted in newer generations of more rapid and accurate molecular assays for the detection, quantification, and genetic characterization of viruses. For this point-counterpoint, we have asked two individuals, Richard L. Hodinka of the Children's Hospital of Philadelphia, a clinical virologist whose laboratory has completely eliminated viral culture in favor of molecular methods, and Laurent Kaiser, head of the Virology Laboratory at the University of Geneva Hospital, who continues to be a strong advocate of viral culture, to discuss the relevance of viral culture in the molecular age.
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机译:长期以来,常规管培养系统一直是临床病毒学中用于从临床标本中生长和鉴定病毒的主要手段。离心增强型小瓶和多孔板培养等创新技术,以及基因工程和混合细胞系的使用,以及对病毒复制的更快检测,即使对于某些生长最缓慢的临床上重要的人类病毒,也都具有合理的周转时间。但是,分子方法,特别是PCR,已经在许多实验室中篡夺了病毒培养的作用,限制了这种传统的病毒检测方法的使用或完全替代了它。随着时间的流逝,分子技术的进步和改进也催生了新一代更快速,更准确的分子检测技术,用于病毒的检测,定量和遗传表征。对于这一点,我们问了两个人:费城儿童医院的Richard L. Hodinka,他是临床病毒学家,其实验室已完全淘汰了病毒培养,而采用了分子方法,他还邀请了Laurent Kaiser,日内瓦大学医院一直是病毒文化的坚定倡导者,他在讨论分子时代病毒文化的重要性。
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