334 Clostridium tyrobutyricum protect intestinal barrier function from LPS-induced apoptosis via p38/JNK signaling pathway in IPEC-J2

摘要

Early weaning, a critical phase in swine production, can compromise intestinal barrier function through mucosal damage and alteration of tight junction integrity. Maintenance of intestinal barrier function plays a pivotal role in optimum gastrointestinal health. This study investigated the effects of Clostridium tyrobutyricum (C.t) on intestinal barrier dysfunction induced by lipopolysaccharide (LPS) and the underlying mechanisms. IPEC-J2 Cells were treated with PBS (the control), LPS (1 μg/mL for 12 h), C.t (10∧8 CFU/mL for 8 h), LPS + C.t (1 μg/mL LPS for 12 h followed by 10∧8 CFU/mL C.t for 8 h), or C.t + LPS (10∧8 CFU/mL C.t for 8 h followed by 1 μg/mL LPS for 12 h). The measurements of trans-epithelium electrical resistance values, tight junction proteins (TJs), apoptotic genes expressions, and MAPK expressions were determined after treatments. Statistical analysis was performed with one-way analysis of variance followed by Duncan multiple range test and differences were considered significant at P <0.05. The results showed that C.t efficiently maintained trans-epithelium electrical resistance values and intestinal permeability after LPS-induced intestinal barrier disruption (P <0.05). The expressions of TJs were enhanced when cells were treated with C.t (P <0.05). Fluorescence imaging and flow cytometry revealed that C.t inhibited cell apoptosis induced by LPS. C.t also increased the relative expression of the anti-apoptotic gene Bcl-2 and decreased that of the apoptotic genes Bax and caspase-3/-8 (P <0.05). The protective effect of C.t on damaged intestinal cell models was associated with suppression of p-p38 and p-JNK. Our findings suggest that C.t could protect against the LPS-induced increase in intestinal permeability and inhibit LPS-induced intestinal apoptosis via the p38/JNK signaling pathway in IPEC-J2 cells. These results suggest C.t is a potential probiotic additive for protecting the intestinal barrier and preventing diarrhea in early weaned piglets.