Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection

摘要

The enteric pathogen is one of the main causes of diarrhea and compromised weight gain in pigs worldwide. Traditional cell-line cultures have been used to study pathogenesis. However, these systems fail to reproduce the epithelial changes observed in the intestines of -infected pigs, specifically, the changes in intestinal cell constitution and gene expression. A more physiologically accurate and state-of-the-art model is provided by swine enteroids derived from stem cell-containing crypts from healthy pigs. The objective of this study was to verify the feasibility of two-dimensional swine enteroids as in vitro models for infection. We established both three- and two-dimensional swine enteroid cultures derived from intestinal crypts. The two-dimensional swine enteroids were infected by in four independent experiments. Enteroid-infected samples were collected 3 and 7 d postinfection for analysis using real-time quantitative PCR and immunohistochemistry. In this study, we show that is capable of infecting and replicating intracellularly in two-dimensional swine enteroids derived from ileum.