Single-cell genomics uncover Pelagibacter as the putative host of the extremely abundant uncultured 37-F6 viral population in the ocean

摘要

Pelagibacter single-amplified genomes (SAGs) analyzed in this study. PCR results and screening of SAGs with vSAG 37-F6-specific primers Seq11 are shown. A PCR band with the expected size was obtained for the Pelagibacter SAG MED40 (lane 2). Lanes 1 and 3 correspond to other SAGs that did not yield positive amplification. Lanes 4 and 5 are negative and positive (DNA template of 37-F6 single-virus MDA product) controls, respectively. Maximum-likelihood phylogeny using a concatenation of 25 conserved proteins from SAGs of this study and from SAR11 representative genomes (see also Supplementary Figure  ). Bootstrap values (%) are indicated at each node ( ). Circles indicate geographical location of the different SAGs analyzed in this study ( )