Positive allosteric modulation of P2X7 promotes apoptotic cell death over lytic cell death responses in macrophages

摘要

J774 macrophages were stimulated with 200 µM ATP in the presence or absence of CK or CK + AZ10606120 (both 10 µM) and Ca -responses were quantified using Fura-2. The sustained response (100-200 sec) was quantified as an indicator of P2X7-dependent responses. J774 macrophages were stimulated with a range of ATP concentrations in the presence or absence of CK (10 µM) prior to quantification of the Fura-2 sustained response as in . J774 macrophages were stimulated with 500 µM ATP in the presence or absence of AZ10606120 (10 µM) to quantify uptake of YOPRO-1 dye. YOPRO-1 dye uptake by J774 macrophages to a range of ATP concentrations in the presence or absence of CK (10 µM). Cell viability of J774 macrophages stimulated with a range of macrophages in the presence or absence of AZ10606120 (10 µM) and quantified using AlamarBlue. Light micrograph demonstrating the morphology of cells treated with 3 mM ATP in the presence or absence of AZ10606120 (10 µM). Experiments are representative of three independent experiments (  = 3). Error bars represent SD. Asterisks represent a significant difference (