Caloric restriction triggers morphofunctional remodeling of astrocytes and enhances synaptic plasticity in the mouse hippocampus

摘要

A mask of astrocytic branches (black) used for Sholl analysis (red circles) was obtained from a maximal intensity projection of z-stack of fluorescence images of an astrocyte loaded with 50 μM Alexa Fluor 594 through a patch pipette. The summary data for the number of intersections of circles with astrocytic branches. Green—CR, black—control (Ctrl). Reconstruction of fluorescence profiles across an astrocyte to estimate VF of optically unresolved thin astrocytic leaflets. Dashed lines indicate the places where the profiles were obtained. The large local increases in fluorescence corresponding to astrocytic branches were cut out. Yellow semicircles indicate the area which was analyzed to avoid boundary effects. The summary data are showing an estimated VF of astrocytic leaflets. The data are presented as mean ± SEM.