Intervention of oncostatin M-driven mucosal inflammation by berberine exerts therapeutic property in chronic ulcerative colitis

摘要

Berberine (50 mg/kg) were orally administrated to DSS-induced UC mice (from day 15 to the end of experiment) and normal control mice were given normal drinking water only. Body weight change (% initial body weight) and disease activity index (DAI) were monitored at the indicated time points. Representative images of colon morphology. Quantitative colon length and the ratio of the colon weight to the length. Serum cytokines determined by Luminex assay. Serum biochemical indicators, including ALB, ALP, and TC. Cytokine secretion levels in full-thickness colon tissue culture. The protein levels of cytokines in colonic homogenates. Representative images of bioluminescent imaging with L-012 and quantitative analysis of positive signals using an IVIS Spectrum CT system. The expression of inflammasome, including NLRP3, ASC, cleaved caspase-1, assayed by western blot and GAPDH was used as a loading control. Data were presented as the mean ± SEM, and  = 15 mice per group. *  p