Genome-scale CRISPR screening for potential targets of ginsenoside compound K

摘要

Representative images of cell state after CK (5 nM) or DMSO treatment for 3 days, with or without pretreatment with 3-MA (5 mM) for 1 day. Scale bar = 150 µm. Quantification of cell numbers in each cellular condition as presented in . Representative EM images of cells after CK (5 nM) or DMSO treatment. Scale bar = 2 µm (left), 0.5 µm (middle), 0.2 µm (right). Arrow head, autolysomes. Quantification of autolysosomes in each cellular condition as presented in . Representative images of cellular localization of RGLC3 probe after CK (5 nm) or DMSO treatment for different times as indicated. Scale bar = 150 µm. Quantification of average dots per cell of mRFP and GFP signals in each cellular condition as presented in . Data are represented as means with SEM. *  P