ADT-OH a hydrogen sulfide-releasing donor induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD

摘要

The chemical structure of ADT-OH. H S production capacity of ADT-OH acting on MEF cells the lead sulfide method. H S measurement released by B16F10 melanoma cells after ADT-OH treatment in different concentration. MEF and B16F10 melanoma cells were incubated with increasing concentrations of ADT-OH for 24 h. Cell viability was determined by CCK-8 assay. B16F10 melanoma cells were treated with ADT-OH at different concentration (0.8–50 μM) and apoptosis was determined by flow cytometry analysis. And quantitative analysis of apoptosis at various concentrations of exposure to ADT-OH. Experiments (  = 3) were performed in triplicate. Representative FACS analysis and quantitative analysis of Annexin V and propidium iodide (PI) staining after ADT-OH treated at different concentration (0.8–50 μM) in MEF cells. Data are represented as mean ± SD for different experiments performed in duplicate. In , *  P c and , *  P