NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma

摘要

NQO1 increases XIAP phosphorylation via AKT activation. Immunoblotting analysis for AKT and phosphor-AKT (pSer473) in NQO1 knock-down/knock-out cells or NQO1 knock-out cells transfection of vector expressing NQO1. Immunoblotting analysis for AKT, phosphor-AKT (pSer473), XIAP, and phosphor-XIAP (pSer87) in NQO1 knock-out cells. NQO1 cells were transfected with plasmid expressing NQO1 and then treated with AKT inhibitor MK2206 (10 μM) for 24 h. Immunoblotting analysis for AKT, phosphor-AKT (pSer473), XIAP and phosphor-XIAP (pSer87) in NQO1 knock-down/knock-out cells transfected with AKT or empty vector. - Trypan blue exclusion assay ( ) and flow cytometry ( ) were performed to analyze the NQO1-depleted cells transfected with AKT. Data are mean ± SEM of  = 3 independent experiments