Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis

摘要

The overall EM density map of a CR subunit. The local resolutions between 4.5 Å and 12.5 Å are color-coded. The CR consists of eight subunits. Each subunit is divided into three overlapping regions for soft mask application during data processing: the Core region, the Nup358-containing region, and the Nup214-containing region. These three regions are indicated by dashed boxes. The individual EM density maps for the Core, Nup358-containing, and Nup214-containing regions. The local resolutions are color-coded identically as in panel ( ). The average resolutions for the Core, Nup358-containing, and Nup214-containing regions are 5.5 Å, 7.1 Å and 7.9 Å, respectively. Shown here are the Fourier shell correlation (FSC) plots of the reconstructions for the three regions. The resolutions were estimated on the basis of the FSC value of 0.143. Cryo-EM reconstruction of the Core region of the CR subunit. Shown here is the EM density map of the Core region (colored yellow), which is placed in the CR subunit (left panel) and displayed in isolation (right panel). Cryo-EM reconstruction of the Nup358-containing region and the Nup214-containing region. Shown here is the EM density map (colored cyan for the Nup358-containing region and green for the Nup214-containing region), which is placed in the CR subunit (left panel) and displayed in isolation (right panel).