Serum IP-10 and IL-17 from Kawasaki disease patients induce calcification-related genes and proteins in human coronary artery smooth muscle cells in vitro

摘要

IP-10/IL-17 secreted in the KD plasma initiates the calcification of HCASMCs. HCASMCs were either used as controls or were treated with either FC or KD plasma for 7, 14, or 21 days; the HCASMCs were either maintained as controls or were treated with IP-10, IL-17, or both, for 7, 14, or 21 days; the HCASMCs were either maintained as controls or were treated with FC or KD plasma, or KD plasma co-mixed with IP-10 and IL-17 blocking antibodies, for 21 days; – the calcification of the HCASMCs was analyzed using ARS stain. All data were mean ± SEM from three independent experiments; *