Identification of new octamer transcription factor 1‐target genes upregulated in castration‐resistant prostate cancer

摘要

Octamer transcription factor 1 ( 1) is an androgen receptor ( )‐interacting partner and regulates the expression of target genes in prostate cancer cells. However, the function of 1 in castration‐resistant prostate cancer ( ) is not fully understood. In the present study, we used 22Rv1 cells as ‐positive model cells to analyze the role of 1 in . We showed that 1 knockdown suppressed cell proliferation and migration of 22Rv1 cells. Using microarray analysis, we identified four and 1‐target genes, disks large‐associated protein 5 ( 5), kinesin family member 15 ( 15), non‐ condensin I complex subunit G ( ), and 80 kinetochore complex component ( 2) in 22Rv1 cells. We observed that knockdown of 5 and 2 suppresses growth and migration of 22Rv1 cells. Furthermore, immunohistochemical analysis showed that positive expression of 5 in prostate cancer specimens is related to poor cancer‐specific survival rates of patients. Notably, enhanced expression of 5 was observed in tissues of patients. Thus, our findings suggest that these four genes regulated by the / 1 complex could have an important role in progression.