MEX3A knockdown inhibits the development of pancreatic ductal adenocarcinoma

摘要

MEX3A is highly expressed in PDA and the construction of MEX3A knockdown cell model. Expression levels of MEX3A in PDA tumor tissues and adjacent normal skin tissues were detected by IHC staining. Kaplan–Meier survival analysis MEX3A expression and overall survival of PDA. MEX3A expression in HPDE6-C7, BxPc-3, PANC-1 and SW1990 cells was detected by qRT-PCR. qRT-PCR was used to screen knockdown efficiency of MEX3A in shMEX3A-1, shMEX3A-2, and shMEX3A-3 groups. Infection efficiency for PANC-1 and SW1990 cells was evaluated by expression of green fluorescent protein 72 h post-infection. , The specificity and validity of the lentivirus-mediated shRNA knockdown of MEX3A expression was verified by qRT-PCR ( ) and western blot analysis ( ). The data were presented as the mean ± SD (n = 3). *P