Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages

摘要

Phosphate deprivation increases /BCG phagocytosis. J774A.1 MOs (5 × 10 ) were incubated with /BCG bacilli labeled red with the PKH-26; nuclei were stained blue with DAPI. Immunofluorescence microscopy shows a marked difference in the degree of phagocytosis between bacilli grown with and without Pi ( , ). For FACS analysis gates considering the percent and the mean fluorescence index (MFI) were carried out ( , ). Dose-dependent phagocytosis that was significantly higher with Pi-deprived mycobacteria was observed ( , ). At a 1:20 MOI, 80.2% of MOs phagocytosed bacilli cultured without Pi while the percent phagocytosis of bacilli cultured with Pi was 38.7;