Increased Interleukin-35 suppresses peripheral CD14+ monocytes function in patients with Kawasaki disease

摘要

Interleukin (IL)-35 concentration in the plasma and IL-35 receptor mRNA relative level in CD14 monocytes in patients with Kawasaki disease (  = 33) and controls (  = 17). Plasma IL-35 concentration was measured by enzyme linked immunosorbent assay. mRNA corresponding to IL-35 receptor subunits, including IL-12Rβ2 and gp130, in purified CD14 monocytes were semi-quantified by real-time reverse transcriptional polymerase chain reaction. IL-35 concentration was elevated in the plasma from patients with Kawasaki disease when compared with controls. There were no significant differences of IL-12Rβ2 or gp130 mRNA relative level between patients with Kawasaki disease and controls. Student’s test was used for comparison. Individual level of each subject was shown. The horizon line presented mean, and error bar presented standard deviation