In vivo model to study the impact of genetic variation on clinical outcome of mastitis in uniparous dairy cows

摘要

Graphical illustration of the intramammary infection (IMI) model. Animals selected for paternal BTA 18 haplotypes favorable (Q,  = 18) or unfavorable (q,  = 18) for somatic cell count received intramammary challenge with (  = 24) or (  = 12) for 96 or 24 h, respectively. During challenge, inoculation was performed in the hind left (HL) and hind right (HR) quarters, and the front left (FL) quarter served as a negative control inoculated with saline solution, while the front right (FR) quarter was not treated. During challenge, inoculation was performed with HR, saline solution was administered HL and front quarters were untreated. Clinical examination, udder examination and blood and milk sampling were performed every 12 h. The graphical illustration of the cow and the udder has been designed by Wolfram Petzl