LPS-treatment of bovine endometrial epithelial cells causes differential DNA methylation of genes associated with inflammation and endometrial function

摘要

LPS effects on DNA methylation in bovine endometrial epithelial cells (bEECs). Principal component analysis displaying overall methylation profiles across all samples. The first dimension explained 21% variation and separated Cow1 from Cow2 and Cow3. The second dimension explained 16% variation, separated both Cow2 versus Cow3. Venn diagram displaying overlapping differentially methylated regions (DMRs) from 24 h sample groups: 0 μg vs. 2 μg (pink), 0 μg vs. 8 μg (blue), and 0 μg vs. 2 μg + 8 μg (green). Heatmap of significant DMRs (1291) showing similar methylation trend for the analyses performed in ( ). The scale shows hypermethylated (red) and hypomethylated (blue) levels for each DMR. Bar plot showing distribution of the percent of hyper and hypomethylated DMRs when comparing time 0 h and 24 h in controls, and 24 h control with 2 μg or 8 μg, and 2 μg + 8 μg combined LPS groups. Top bar shows a similar pattern for total DMRs identified in 2 μg, 8 μg, and 2 μg + 8 μg analysis