Short paired-end reads trump long single-end reads for expression analysis

摘要

Spearman’s rank correlations for kallisto-derived transcripts per million (TPM) between the gold standard paired-end 2 × 125 strategy and alternative strategies. Violin plots of ( ) transcript and ( ) gene-level inference. Comparison of correlations with 2 × 125 between 2 × 40 and 1 × 75 for ( ) transcript and ( ) genes, and between 2 × 40 and 1 × 125 for ( ) transcript and ( ) genes. For - , symbol colors correspond to SRA accessions, and points above the red dotted line are samples where estimates of expression from 2 × 40 is more highly correlated with the gold standard than the contrasted single-end strategy