Novel optogenetics tool: Gt_CCR4 a light-gated cation channel with high reactivity to weak light

摘要

A phylogenetic tree of two families of cation channelrhodopsins (CCRs) and proton pumps. Chlorophyte CCRs shown are from ( ChR1, ChR2) ( _ChR1, ChR2), ( _ChR2), ( _ChR). Cryptophyte DTD CCRs are from ( _CCR1–4). H pumps shown are from (GR), C-169 (CsR), (BR), and (AR3). Amino acid alignments of bacteriorhodopsin (BR), _ChR2, and CCR4. The characteristic of amino acids in BR, _ChR2, and _CCR4 were listed. In addition, amino acid numbers of each protein and transmembrane helix (TM) numbers are indicated