Polarimetric second-harmonic generation microscopy of the hierarchical structure of collagen in stage I-III non-small cell lung carcinoma

摘要

Polarimetric second-harmonic generation (P-SHG) microscopy is used to quantify the structural alteration of collagen in stage-I,-II and -III non-small cell lung carcinoma (NSCLC) tissue. The achiral and chiral molecular second-order susceptibility tensor components ratios ( and , respectively), the degree of linear polarization ( ) and the in-plane collagen fiber orientation ( ) were extracted. Further, texture analysis was performed on the SHG intensity, , , and . The distributions of , , and as well as the textural features of entropy, correlation and contrast show significant differences between normal and tumor tissues.