Fluorescence guided neurosurgery based on 5-aminolevulinic acid (5-ALA) has significantly increased maximal safe resections. Fluorescence lifetime imaging (FLIM) of 5-ALA could further boost this development by its increased sensitivity. However, neurosurgeons require real-time visual feedback which was so far limited in dual-tap CMOS camera based FLIM. By optimizing the number of phase frames required for reconstruction, we here demonstrate real-time 5-ALA FLIM of human high- and low-grade glioma with up to 12 Hz imaging rate over a wide field of view (11.0 x 11.0 mm). Compared to conventional fluorescence imaging, real-time FLIM offers enhanced contrast of weakly fluorescent tissue.
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机译：基于5-氨基乙酰丙酸（5-ALA）的荧光引导神经外科手术显着提高了最大的安全切除率。 5-ALA的荧光寿命成像（FLIM）可以通过提高灵敏度来进一步促进这一发展。但是，神经外科医师需要实时的视觉反馈，这在基于双头CMOS摄像头的FLIM中受到了限制。通过优化重建所需的相架数量，我们在此演示了人类高级别和低级别神经胶质瘤的实时5-ALA FLIM，在宽视场（11.0 x 11.0 mm）下的成像速率高达12 Hz。与传统的荧光成像相比，实时FLIM可以增强弱荧光组织的对比度。
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