Identification of pAKT as a pharmacodynamic marker for MER kinase in human melanoma G361 cells

摘要

GAS6 induces pAKT in human melanoma G361 cells. Western blot showing the expression of AXL, MER, and TYRO3 among 9 different human cancer cell lines. and High-content imaging assay showing robust phosphorylated AKT (pAKT) activation upon growth arrest–specific protein 6 (GAS6) stimulation. GAS6-activated pAKT can be reversed by AXL/MER inhibitor 3 in G361 cells. Three independent experiments were conducted. Results are shown as mean ± standard deviation (SD) of 3 wells from a representative experiment (*