Human bone morphogenetic protein-2 (hBMP-2) characterization by physical–chemical immunological and biological assays

摘要

SDS-PAGE analysis run in 15% acrylamide gel, stained with Coomassie Blue G, of: (MM) molecular mass markers; (1) -derived met-hBMP-2 (GenScript) 1 µg, run under non-reducing conditions; (2) CHO-derived hBMP-2 (Infuse from Medtronic) 1 µg, run under reducing conditions; (3) same sample as in 2, run under non-reducing conditions; (4) same sample as in 1, run under reducing conditions. Western blot analysis of: (1) -derived met-hBMP-2, 0.1 µg, run under non-reducing conditions; (2) CHO-derived hBMP-2 (Infuse ), 0.25 µg, run under reducing conditions; (3) same sample as in 2, run under non-reducing conditions; (4) same sample as in 1, run under reducing conditions