首页> 美国卫生研究院文献>Journal of Clinical Microbiology >GlpQ: an antigen for serological discrimination between relapsing fever and Lyme borreliosis.
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GlpQ: an antigen for serological discrimination between relapsing fever and Lyme borreliosis.

机译:GlpQ:一种用于在复发性发热和莱姆疏螺旋体病之间进行血清学鉴别的抗原。

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摘要

Tick-borne relapsing fever is caused by numerous Borrelia species maintained in nature by Ornithodoros tick-mammal cycles. Serological confirmation is based on either an immunofluorescence assay or an enzyme-linked immunosorbent assay using whole cells or sonicated Borrelia hermsii as the antigen. However, antigenic variability of this bacterium's outer surface proteins and antigens shared with the Lyme disease spirochete (B. burgdorferi), may cause both false-negative and false-positive results when testing sera of patients suspected to have either relapsing fever or Lyme disease. To develop a specific serological test for relapsing fever, we created a genomic DNA library of B. hermsii, screened transformed Escherichia coli cells for immunoreactivity with high-titered (> or = 1:2,048) human anti-B. hermsii antiserum, and selected an immunoreactive clone (pSPR75) expressing a 39-kDa protein. DNA sequencing, subcloning, and serum adsorption experiments identified the immunoreactive protein as a homolog of GlpQ, a glycerophosphodiester phosphodiesterase identified previously in E. coli, Haemophilus influenzae, and Bacillus subtilis. Serum samples from humans and mice infected with B. hermsii or other species of relapsing fever spirochetes contained antibodies recognizing GlpQ, whereas serum samples from Lyme disease and syphilis patients were nonreactive. Serologic tests based on this antigen will identify people exposed previously to relapsing fever spirochetes and help clarify the distribution of relapsing fever and Lyme disease in situations in which the occurrence of their causative agents is uncertain.
机译:Or传播的热是由鸟粪tick哺乳动物周期在自然界维持的多种疏螺旋体引起的。血清学确认是基于免疫荧光测定或酶联免疫吸附测定,使用全细胞或超声波氏疏螺旋体作为抗原。但是,当测试怀疑患有复发性发热或莱姆病患者的血清时,该细菌的外表面蛋白和莱姆病螺旋体(B. burgdorferi)共有的抗原的抗原变异性可能会导致假阴性和假阳性结果。为了开发针对复发性发热的特定血清学测试,我们创建了赫氏芽孢杆菌的基因组DNA库,筛选了转化的大肠杆菌细胞与高滴度(>或= 1:2,048)人源抗B的免疫反应性。 hermsii抗血清,并选择了一种表达39 kDa蛋白的免疫反应性克隆(pSPR75)。 DNA测序,亚克隆和血清吸附实验将免疫反应蛋白鉴定为GlpQ的同源物,GlpQ是先前在大肠杆菌,流感嗜血杆菌和枯草芽孢杆菌中鉴定的甘油磷酸二酯磷酸二酯酶。来自感染了疱疹双歧杆菌或其他复发性发热螺旋体的人和小鼠的血清样品含有识别GlpQ的抗体,而来自莱姆病和梅毒患者的血清样品则没有反应。基于这种抗原的血清学检测将确定以前接触过复发性发烧螺旋体的人,并有助于弄清在不确定其致病因素发生的情况下复发性发烧和莱姆病的分布。

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