Characterization and Resolution of Evaporation-Mediated Osmolality Shifts that Constrain Microfluidic Cell Culture in Poly(dimethylsiloxane) Devices

摘要

Evaporation is a critical problem when handling sub-microliter volumes of fluids. This paper characterizes this problem as it applies to microfluidic cell culture in poly(dimethylsiloxane) (PDMS) devices and provides a practical solution. Evaporation-mediated osmolality shifts through PDMS membranes with varying thicknesses (10, 1, 0.2 or 0.1 mm) were measured over 96 hours. Even in humidified cell culture incubators, evaporation through PDMS and associated shifts in the osmolality of culture media was significant and prevented mouse embryo and human endothelial cell growth and development. A simple diffusion model, where the measured diffusion coefficient for PDMS matches reported values of ~10⁻⁹ m²/s, accounts for these evaporation and osmolality shifts. To overcome this problem a PDMS-parylene-PDMS hybrid membrane was developed that greatly suppresses evaporation and osmolality shifts, yet possesses thinness and the flexibility necessary to interface with deformationbased microfluidic actuation systems, maintains the clarity for optical microscopy, and enables the successful development of single cell mouse embryos into blastocysts under static conditions and culture of human endothelial cells underdynamic recirculation of sub-microliter volumes of media. These insights and methods demonstrated specifically for embryo and endothelial cell studies will be generally useful for understanding and overcoming evaporation-associated effects in microfluidic cell cultures.