A high-throughput 3-parameter flow cytometry-based cell death assay

摘要

Apoptosis plays a role in many disease states, and the evaluation of novel therapeutics that alter the apoptotic cascade is an area of intense investigation. However, many generally available methods to evaluate cell death are either time consuming, imprecise, or both. We report a novel system that permits simultaneous evaluation of three apoptotic markers (cell membrane integrity, mitochondrial membrane potential, and cell cycle progression) with minimal technical manipulation. This system is particularly well-suited for initial efficacy and toxicology profiling of new candidate apoptosis-inducing or apoptosis-inhibiting agents.