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Laser-induced collagen remodeling and deposition within the basilar membrane of the mouse cochlea

机译:激光诱导的胶原重塑和沉积在小鼠耳蜗基底膜内

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摘要

The cochlea is the mammalian organ of hearing. Its predominant vibratory element, the basilar membrane, is tonotopically tuned, based on the spatial variation of its mass and stiffness. The constituent collagen fibers of the basilar membrane affect its stiffness. Laser irradiation can induce collagen remodeling and deposition in various tissues. We tested whether similar effects could be induced within the basilar membrane. Trypan blue was perfused into the scala tympani of anesthetized mice to stain the basilar membrane. We then irradiated the cochleas with a 694-nm pulsed ruby laser at 15 or 180 J /cm2. The mice were sacrificed 14 to 16 days later and collagen organization was studied. Polarization microscopy revealed that laser irradiation increased the birefringence within the basilar membrane in a dose-dependent manner. Electron microscopy demonstrated an increase in the density of collagen fibers and the deposition of new fibrils between collagen fibers after laser irradiation. As an assessment of hearing, auditory brainstem response (ABR) thresholds were found to increase moderately after 15 J/cm2 and substantially after 180 J /cm2. Our results demonstrate that collagen remodeling and new collagen deposition occurs within the basilar membrane after laser irradiation in a similar fashion to that found in other tissues.
机译:耳蜗是哺乳动物的听力器官。根据其质量和刚度的空间变化,对它的主要振动元件基底膜进行了局部地调谐。基底膜的组成胶原纤维影响其硬度。激光照射可以诱导胶原重塑和沉积在各种组织中。我们测试了是否可以在基底膜内诱发类似的作用。锥虫蓝被灌注到麻醉小鼠的结ala中以染色基底膜。然后,我们用694 nm脉冲红宝石激光以15或180 J / cm 2 照射耳蜗。 14至16天后处死小鼠,研究胶原蛋白的组织。偏振显微镜显示,激光辐照以剂量依赖性方式增加了基底膜内的双折射。电子显微镜表明,在激光辐照后,胶原纤维的密度增加并且胶原纤维之间新的原纤维沉积。作为听力评估,发现听觉脑干反应(ABR)阈值在15 J / cm 2 之后适度增加,而在180 J / cm 2 之后显着增加。我们的结果表明,在激光照射后,基底膜内会发生胶原重塑和新的胶原沉积,其发生方式与在其他组织中相似。

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