Synergy within structural biology of single crystal optical spectroscopy and x-ray crystallography

摘要

Advances in the adaptation of optical spectroscopy to monitor photo-induced or enzyme catalyzed reactions in the crystalline state have enabled x-ray crystal structures to be accurately linked with spectroscopically defined intermediates. This in turn has led to a deeper understanding of the role protein structural changes play in function. The integration of optical spectroscopy with x-ray crystallography is growing, and now extends beyond linking crystal structure to reaction intermediate. Recent examples of this synergy include applications in protein crystallization, x-ray data acquisition, radiation damage and acquisition of phase information important for structure determination.