Porphyromonas gingivalis lipopolysaccharide induces tumor necrosis factor-α and interleukin-6 (IL-6) secretion and CCL25 gene expression in mouse primary gingival cell lines: IL-6-driven activation of CCL2

摘要

Background and ObjectivePorphyromonas gingivalis infection is strongly associated with periodontitis. Although P. gingivalis is known to elicit a strong inflammatory response, details of that remain fragmentary. To understand the local response to P. gingivalis, primary cell lines derived from mouse gingival tissues were exposed to P. gingivalis or E. coli lipopolysaccharide (LPS), and cytokine production for interleukin-6 (IL-6) and tumor necrosis factor-α (TNFα were measured. CCL25 gene expression was measured by real-time PCR. Cells stimulated with combinations of IL-6, soluble IL-6 receptor (sIL-6R), and/or soluble gp130 (sgp130) were assayed for CCL2 and TNFα secretion.