Two-Step Purification of Cordycepin from Cordyceps Millitaris by High-Speed Countercurrent Chromatography

摘要

Cordycepin is successfully isolated and purified from Cordyceps millitaris in two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate–1-butanol–water (3:2:5, v/v/v) and trichloromethane–methanol–1-butanol–water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatography analysis.