A rapid and simple plasmid isolation procedure was developed for the epidemiological analysis of plasmid-mediated antimicrobial resistance. By this method, plasmid DNAs ranging in molecular weight between 2.0 and 122 X 10(6) could be detected. Various bacteria, such as strains of the family Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus influenzae, and Staphylococcus aureus, could be analyzed. The plasmid DNA obtained could be directly used for restriction endonuclease analysis without further purification. In addition, this method made it possible to analyze several cultures at the same time.
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机译：开发了一种快速，简单的质粒分离方法，用于质粒介导的抗药性的流行病学分析。通过这种方法，可以检测到分子量在2.0到122 X 10（6）之间的质粒DNA。可以分析各种细菌，例如肠杆菌科，铜绿假单胞菌，流感嗜血杆菌和金黄色葡萄球菌的菌株。获得的质粒DNA无需进一步纯化即可直接用于限制性核酸内切酶分析。此外，这种方法还可以同时分析几种文化。
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