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Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.

机译:高效液相色谱法分析厌氧菌中的短链酸。

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摘要

A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.
机译:使用Aminex HPX-87色谱柱通过高效液相色谱解析25种短链脂肪酸的标准混合物。厌氧菌在培养基中产生的酸在用乙醚萃取并重新萃取到少量0.1 N NaOH中后,通过高效液相色谱进行分析。通过高效液相色谱柱中捕获的洗脱液馏分的气相色谱-质谱分析,确认了厌氧链球菌培养提取物中富马酸的存在。

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