A sequence element that tunes E. coli tRNAGGCAla to ensure accurate decoding

摘要

Mutating the rare A32-U38 nucleotide pair at the top of the anticodon loop of E. coli tRNAGGC^Ala to a more common U32-A38 pair results in a tRNA that performs almost normally on cognate codons but is unusually efficient in reading near-cognate codons. Pre-steady state kinetic measurements on E. coli ribosomes show that unlike the wild-type tRNAGGC^Ala, the misreading mutant tRNAGGC^Ala shows rapid GTP hydrolysis and no detectable proofreading on near-cognate codons. Similarly, tRNAGGC^Ala mutated to contain C32-G38, a pair which is found in some bacterial tRNAGGC^Ala sequences, was able to decode only the cognate codons, while tRNAGGC^Ala containing a more common C32-A38 pair was able to decode all cognate and near-cognate codons tested. We propose that many of the phylogenetically conserved sequence elements present in each tRNA have evolved to suppress translation of near-cognate codons.