Semi-continuous Flow Electroporation Chip for High Throughput Transfection on Mammalian Cells

摘要

We have recently developed a semi-continuous flow electroporation (SFE) device for in vitro DNA delivery. Cells mixed with plasmid DNA continuously flowed through a serpentine channel, the side walls of which also serving as electrodes. Using pWizGFP plasmid and K562 cells as a model system, SFE showed better transgene expression (10~15%) compared to a commercial electroporation system. Quantitative results via MTS assay also revealed a 50% or higher cell viability. Similar observations were also found with pWizGFP transfection to mouse embryonic stem cells. Such improvements were attributed to less gas formation and joule heating in SFE.