The Structure and NO binding Properties of the Nitrophorin-like Heme-binding protein from Arabidopsis thaliana gene locus At1g79260.1

摘要

The protein from Arabidopsis thaliana gene locus At1g79260.1 is comprised of 166-residues and is of previously unknown function. Initial structural studies by the Center for Eukaryotic Structural Genomics (CESG) suggested that this protein might bind heme, and consequently, the crystal structures of apo and heme-bound forms were solved to near atomic resolution of 1.32 Å and 1.36 Å respectively. The rate of hemin loss from the protein was measured to be 3.6 × 10⁻⁵ s⁻¹, demonstrating that it binds heme specifically and with high affinity. The protein forms a compact ten-stranded β-barrel that is structurally similar to the lipocalins and fatty acid binding proteins (FABPs). One group of lipocalins, the nitrophorins (NP), are heme proteins involved in nitric oxide (NO) transport and show both sequence and structural similarity to the protein from At1g79260.1 and two human homologues, all of which contain a proximal histidine capable of coordinating a heme iron. Rapid-mixing and laser photolysis techniques were used to determine the rate constants for carbon monoxide (CO) binding to the ferrous form of the protein (k'CO = 0.23 µM⁻¹s⁻¹, kCO = 0.050 s⁻¹) and NO binding to the ferric form ( k'NO = 1.2 µM⁻¹ s⁻¹, kNO = 73 s⁻¹). On the basis of both structural and functional similarity to the nitrophorins, we have named the protein nitrobindin and hypothesized that it plays a role in NO transport. However, one of the two human homolog of nitrobindin contains a THAP domain, implying a possible role in apoptosis.