ATP production rate via CK or ATP synthase in vivo: a novel superfast magnetization saturation transfer method

摘要

Rationale and Objective³¹P magnetization saturation transfer (MST) experiment is the most widely used method to study ATP metabolism kinetics. However, its lengthy data acquisition time greatly limits the wide biomedical applications in vivo, especially for studies requiring high spatial and temporal resolutions. We aim to develop a novel superfast MST method that can accurately quantify ATP production rate constants (kf) through creatine kinase (CK) or ATP synthase (ATPase) with two spectra.