A Novel In Vitro Assay to Assess Phosphorylation of 3′-18Ffluoro-3′-Deoxythymidine

摘要

Purpose3′-[¹⁸F]fluoro-3’-deoxythymidine ([¹⁸F]FLT) is phosphorylated by thymidine kinase 1 (TK-1), a cell cycle regulated enzyme. Appropriate use of [¹⁸F]FLT tracer requires validation of the TK-1 activity. Here, we report development of a novel phosphoryl-transfer assay to assess phosphorylation of [¹⁸F]FLT both in tumor cell lysates and tumor cells.