Fluorescent Liposomes for Differential Interactions with Glycosaminoglycans

摘要

We have successfully synthesized a lipid containing the pyranine dye as the hydrophilic head group. This lipid was incorporated into liposomes with 1-palmitoyl-sn-glycero-3-phosphocholine as the major component. The resultant liposomes displayed differential enhancements in fluorescence emission intensity in the presence of nanomolar concentrations of different glycosaminoglycans. Linear discriminant analysis of the fluorescence response data demonstrate that the liposomes are able to distinguish between different GAGs. In addition, we also demonstrate that the liposomes incorporating the pyranine lipid are able to distinguish between dilute serum from healthy individuals and serum containing elevated chondroitin sulfate (simulated serum from an Alzheimer’s disease patient).