Regulation of basal LC20 phosphorylation by MYPT1 and CPI-17 in murine gastric antrum gastric fundus and proximal colon smooth muscles

摘要

BackgroundMyosin light chain kinase (MLCK) and phosphatase (MLCP) govern myosin light chain (LC20) phosphorylation and smooth muscle contraction. Rho kinase (ROK) inhibits MLCP, resulting in greater LC20 phosphorylation and force generation at a given [Ca²⁺]i. Here, we investigate the role of ROK in regulating LC20 phosphorylation and spontaneous contractions of gastric fundus, gastric antrum, and proximal colon smooth muscles.