Azide-Reactive Liposome for Chemoselective and Biocompatible Liposomal Surface Functionalization and Glyco-Liposomal Microarray Fabrication

摘要

A chemically selective liposomal surface functionalization and liposomal microarray fabrication using azide-reactive liposome are described. First, liposome carrying PEG-triphenylphosphine was prepared for Staudinger ligation with azide-containing biotin, which was conducted in PBS buffer (pH 7.4) at room temperature without catalyst. Then, immobilization and microarray fabrication of the biotinylated liposome onto streptavidin-modified glass slide via specific streptavidin/biotin interaction were investigated by comparing with direct-formed biotin-liposome, which was prepared by conventional liposome formulation of lipid-biotin with all other lipid components. Next, covalent microarray fabrication of liposome carrying triphenylphosphine onto an azide-modified glass slide and its further glyco-modification with azide-containing carbohydrate were demonstrated for glyco-liposomal microarray fabrication via Staudinger ligation. Fluorescence imaging confirmed the successful immobilization and protein binding of the intact immobilized liposomes and arrayed glyco-liposomes. The azide-reactive liposome provides a facile strategy for a membrane-mimetic glycoarray fabrication, which may find important biological and biomedical applications such as studying carbohydrate-protein interaction and toxin and antibody screening.