Determination of glycation sites by tandem mass spectrometry in a synthetic lactose-bovine serum albumin conjugate a vaccine model prepared by dialkyl squarate chemistry

摘要

RATIONALENeoglycoconjugate vaccines synthesized by the squaric acid spacer method allow single point attachment of the carbohydrate antigen to the protein carrier. However, the localization of the carbohydrate antigen sites of conjugation on the protein carrier has been an elusive task difficult to achieve.